A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

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9.4K Views

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10:28 min

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August 17th, 2019

DOI :

10.3791/60017-v

August 17th, 2019

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Laszlo Radnai¹^,², Rebecca F. Stremel¹^,², James R. Sellers³, Gavin Rumbaugh², Courtney A. Miller¹^,²

¹Department of Molecular Medicine, The Scripps Research Institute, ²Department of Neuroscience, The Scripps Research Institute, ³Laboratory of Molecular Physiology, NHLBI, National Institutes of Health

Podsumowanie

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NADH Coupled ATPase Assay

Semi high throughput Screening

ATPase Inhibitors

Non muscle Myosin II Inhibitors

Methamphetamine Use Disorder

Actin Solution Preparation

Enzyme Mix

Substrate Mix

Calibration Dilutions

Positive And Negative Controls

Assay Optimization

Substrate Specificity

Rozdziały w tym wideo

0:04

Title

0:46

Measuring ATPase Activities and Inhibitory Effects of Small Molecule Inhibitors

5:48

Analyzing Data

7:54

Results: Fluorescence Intensity

9:26

Conclusion

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