Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

0:43

Preparation of Cultured Cells

1:23

Treatment of Cells and Photocrosslinking

4:34

Attachment of Fluorescent Tag by Click Chemistry for Visualization of Labeled Proteins

5:48

Attachment of Biotin Tag by Click Chemistry for Affinity Purification of Labeled Proteins

8:54

Results: Live-cell Photoaffinity Labeling Identifies a Major Itraconazole-binding Protein

10:14

Conclusion