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Nanyang Technological University

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

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7.6K Views

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09:23 min

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June 29th, 2018

DOI :

10.3791/57240-v

June 29th, 2018

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Huan Chen*¹^,², Ian Arthur Palmer*¹, Jian Chen¹^,², Ming Chang¹^,², Stephen L. Thompson³, Fengquan Liu², Zheng Qing Fu¹

¹Department of Biological Sciences, University of South Carolina, ²Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, ³Department of Instructions and Teacher Education, University of South Carolina

Podsumowanie

Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.

Przeglądaj więcej filmów

Citrus Greening

Candidatus Liberibacter Spp

Citrus Leaf Tissue

Pathogen Identification

Quantitative Analysis

Laboratory Procedure

DNA Precipitation

Rozdziały w tym wideo

0:04

Title

0:49

Isolation of Genomic DNA from Plant Tissue

5:04

Perform PCR on Genomic DNA Samplesand Electrophoresis of the Amplified DNA II. Isolation of Genomic DNA from Plant Tissueon an Agarose Gel

7:34

Results: PCR Amplification of Genomic DNA to Identify the Citrus Greening Bacterium

8:52

Conclusion

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