Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

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7.6K Views

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09:23 min

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June 29th, 2018

DOI :

10.3791/57240-v

June 29th, 2018

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Huan Chen*¹^,², Ian Arthur Palmer*¹, Jian Chen¹^,², Ming Chang¹^,², Stephen L. Thompson³, Fengquan Liu², Zheng Qing Fu¹

¹Department of Biological Sciences, University of South Carolina, ²Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, ³Department of Instructions and Teacher Education, University of South Carolina

Podsumowanie

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Keywords Citrus Greening

Candidatus Liberibacter Spp

Citrus Leaf Tissue

PCR Detection

Pathogen Identification

Citrus Farming

Quantitative Analysis

Rozdziały w tym wideo

0:04

Title

0:49

Isolation of Genomic DNA from Plant Tissue

5:04

Perform PCR on Genomic DNA Samplesand Electrophoresis of the Amplified DNA II. Isolation of Genomic DNA from Plant Tissueon an Agarose Gel

7:34

Results: PCR Amplification of Genomic DNA to Identify the Citrus Greening Bacterium

8:52

Conclusion

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