Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

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14.2K Views

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13:17 min

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January 28th, 2016

DOI :

10.3791/52894-v

January 28th, 2016

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Eric Ehrke-Schulz¹, Wenli Zhang¹, Maren Schiwon¹, Thorsten Bergmann¹, Manish Solanki¹, Jing Liu¹, Philip Boehme¹, Theo Leitner¹, Anja Ehrhardt¹

¹Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, University Witten/Herdecke

Podsumowanie

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Keywords Adenoviral Vector

Rozdziały w tym wideo

0:05

Title

1:14

Pre-Amplification of HCAdV Vectors in the Producer Cell Line 116

5:00

Large Scale Amplification of High-capacity Adenovirus Vectors in 116 Cells in Suspension

8:49

Purification of High-capacity Adenovirus

11:58

Results: Characterization of the Final Virus Preparations

12:34

Conclusion

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