Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

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7.7K Views

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11:01 min

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September 25th, 2016

DOI :

10.3791/52224-v

September 25th, 2016

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Bradley P. Weegman¹, Ahmad Essawy², Peter Nash², Alexandra L. Carlson², Kristin J. Voltzke³, Zhaohui Geng², Marjan Jahani², Benjamin B. Becker², Klearchos K. Papas⁴, Meri T. Firpo²

¹Radiology, University of Minnesota, ²Medicine, University of Minnesota, ³School of Public Health, University of Minnesota, ⁴Surgery, University of Arizona

Podsumowanie

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Keywords Nutrient Regulation

Continuous Feeding

Large scale Cell Expansion

Rozdziały w tym wideo

0:05

Title

1:05

Assemble the Continuous Feeding System

4:30

Spheroid Formation

6:04

Continuous Feeding Culture and Adjusted Feed Rate

8:50

Results: Improved Growth Curve and Cell Yields from Cells Grown in Stirred Suspension Bioreactors

10:05

Conclusion

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