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JoVE Journal
Biology
This content is Open Access.
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
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09:01 min
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March 16th, 2011
DOI :
March 16th, 2011
•Rozdziały w tym wideo
0:05
Title
1:38
Creation of a Mutant Library: Mutagenesis
3:23
Creation of a Mutant Library: Readout
4:03
Mutant Identification and Characterization: Construction of Gradient
5:06
Mutant Identification and Characterization: Stamp Transfer of Bacteria
6:27
Mutagenesis and Functional Selection Protocols: Representative Results
8:14
Conclusion
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