Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

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8.8K Views

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10:27 min

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December 5th, 2019

DOI :

10.3791/60533-v

December 5th, 2019

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Joanna Kud¹, Wenjie Wang², Yulin Yuan², Allan Caplan², Joseph C. Kuhl², Louise-Marie Dandurand¹, Fangming Xiao²

¹Department of Entomology, Plant Pathology and Nematology, University of Idaho, ²Department of Plant Sciences, University of Idaho

Podsumowanie

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RING type E3 Ubiquitin Ligases

Enzymatic Activity

Site directed Mutagenesis

Biochemical Characterization

Stress Signaling

Homeostasis

Cys And His Amino Acids

E Coli Transformation

Ubiquitination Reaction

SDS PAGE Loading Buffer

Protein Transfer

Rozdziały w tym wideo

0:04

Title

1:04

Site-directed Mutagenesis

2:29

Recombinant Protein Purification and In Vitro Ubiquitination Assay

3:40

Agrobacterium-mediated Transient Protein Expression in Nicotiana benthamiana Leaves and In Planta Ubiquitination Assay

6:36

Establishing the Link Between Enzymatic Activity and Function In Planta

7:27

Results: In Vitro and In Planta Ubiquitination Assay

9:20

Conclusion

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