JoVE Journal

Neuroscience

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Nanyang Technological University

マウス脳スライスの準備中に発作のような活動の直流刺激とマルチ電極アレイ録音

Studies have shown that cathodal transcranial direct-current stimulation can produce suppressive effects on drug-resistant seizures. In this study, an in vitro experimental setup was devised in which the direct-current stimulation and multielectrode array recording of seizure-like activity were evaluated in mice brain slice preparation. The direct-current stimulation parameters were evaluated.

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Title

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Preparation of Medial Thalamus-anterior Cingulate Cortex Brain Slice

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Preparation of Perfusion Chamber for Multielectrode Array Recording and Electric Field Generation

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Electrically-induced Cortical Synaptic Responses and Seizure-like Activity

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Testing Effect of DCS on Evoked Cortical Responses

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Results: Testing of Evoked Responses and Effect of DCS on Seizure Activity

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Conclusion

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