גישה G-quadruplex זיקה-DNA עבור טיהור של enzymatically Active G4 Resolvase1

0:54

Lyse Bacterial Pellets Expressing Human Recombinant G4 Resolvase1 (rG4R1)

3:06

Prepare G4-magnetic Beads

4:07

Bind Histidine-tagged rG4R1 to Cobalt Beads

5:47

Elute rG4R1 from Cobalt Beads

7:07

Bind rG4R1 to G4-bound SPB and Elute in an ATP-dependent Manner

8:55

Results: Analysis and Quantification of Catalytically Active, Purified rG4R1

10:06

Conclusion