JoVE Journal

Immunology and Infection

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Nanyang Technological University

פיתוח מודל קוליטיס מונחה Antigen ללמוד מצגת של אנטיגנים על ידי אנטיגן הצגת תאים לתאי T

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

Chapters in this video

0:05

Title

1:28

Colonic Lamina propria Cell Isolation

3:58

Results: Host CX3CR1+ Macrophages Communicate with Donor OT-II Cells During OVA-driven Transfer Colitis

6:03

Conclusion

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