Extrémité 3' séquençage bibliothèque préparation avec A-seq2

0:46

Isolation of mRNA from Cells

3:24

5' End Phosphorylation, DNase Treatment, and 3' End Blocking

4:30

Ligation of Reverse 3' Adapters to 5' End of RNA Fragments and Reverse Transcription

5:59

Digestion with Uracil DNA Glycosylase Enzyme Mix and Ligation of 5' Adapters to 5' Ends of cDNA

7:44

Pilot PCR, Amplification of Libraries, and Size Selection

9:21

Computational Analysis of DNA Sequence Data

10:03

Results: The A-seq2 Method Maps pre-mRNA 3' End Processing Sites

10:52

Conclusion