Chapter 15
Mass Spectrometry Fragmentation Methods
Espectrometría de masas: fragmentación de alcanos de cadena larga
The molecular ions of linear alkanes prefer to fragment at the carbon-carbon bond away from the end of the chain since the cleavage of an inner bond ...
Espectrometría de masas: fragmentación de alcanos ramificados
This lesson delves into the mass spectrometry of branched alkane fragmentation. Branched alkanes possess secondary or tertiary carbon atoms, which ...
Espectrometría de masas: fragmentación de cicloalcanos
In mass spectrometry, cycloalkanes exhibit distinct fragmentation patterns due to the inherent stability of their molecular ions compared to linear or ...
Espectrometría de masas: fragmentación de alquenos
Alkenes lose one electron from the unsaturated π bond upon ionization and form stable molecular ions. Further fragmentation of alkenes occurs through ...
Espectrometría de masas: fragmentación de cicloalquenos
The molecular ions of cycloalkenes undergo fragmentation via a retro-Diels–Alder reaction.
The reaction proceeds via the cleavage of two ...
Espectrometría de masas: fragmentación de alquinos
The fragmentation of alkynes preferentially occurs at the carbon–carbon bond between the α and β carbon of the alkyne bond to generate a ...
Espectrometría de masas: fragmentación del alcohol
Alcohols (R-OH) ionize to lose one non-bonded electron from the oxygen atom, forming molecular ions. Due to their tendency to fragment rapidly, the ...
Espectrometría de masas: fragmentación de compuestos aromáticos
Upon ionization, aromatic compounds generate a molecular ion that is observed as a prominent peak in their mass spectra. For example, the molecular ion ...
Espectrometría de masas: fragmentación de aminas
Amines can be identified using mass spectroscopy based on their characteristic fragmentation patterns. The molecular ions of amines undergo fragmentation ...
Mass Spectrometry: Alkyl Halide Fragmentation
Chlorine isotopes exist as 35Cl and 37Cl in a 3:1 ratio, while bromine isotopes exist as 79Br and 81Br in a 1:1 ratio. The mass spectrum of alkyl halides ...
Mass Spectrometry: Alkyl Halide Fragmentation
Chlorine isotopes exist as 35Cl and 37Cl in a 3:1 ratio, while bromine isotopes exist as 79Br and 81Br in a 1:1 ratio. The mass spectrum of alkyl halides ...
Espectrometría de masas: fragmentación de aldehídos y cetonas
In mass spectrometry, the fragmentation of aliphatic aldehydes and ketones generally occurs through three key mechanisms: α-cleavage, inductive ...
Espectrometría de masas: fragmentación de ácido carboxílico, éster y amida
The fragmentation patterns observed for compounds such as carboxylic acids, esters, and amides in the mass spectra include ⍺-cleavage and McLafferty ...
Espectrometría de masas de ionización química (CI)
The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ...
Espectrometría de masas de ionización por electrospray (ESI)
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional ...
Ionización por desorción láser asistida por matriz (MALDI)
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and ...
ACERCA DE JoVE
Copyright © 2025 MyJoVE Corporation. Todos los derechos reservados