The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
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12:15 min
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October 3rd, 2017
DOI :
October 3rd, 2017
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Chapters in this video
0:05
Title
1:24
Introduction to the Accompanying Publication
2:47
Nematode Fixation
4:19
Nematode Antibody Staining
6:33
Applying RNAi to Nematodes Using a Feeding Protocol
7:57
Analyzing Phenotypes Using Fluorescence Dissecting Microscopy
8:41
Analyzing Phenotypes Using Confocal Microscopy
10:14
Results: Immunohistochemistry, RNAi and Microscopic Analysis of Intestinal Apical membrane and Lumen Morphogenesis
11:45
Conclusion
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