CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

9.4K Views

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09:39 min

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June 9th, 2018

DOI :

10.3791/57311-v

June 9th, 2018

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Weijun Feng*¹, Lena Herbst*², Peter Lichter², Stefan M. Pfister³^,⁴^,⁵, Hai-Kun Liu¹, Daisuke Kawauchi³^,⁴

¹Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, ²Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), ³Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), ⁴Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), ⁵Department of Pediatric Hematology and Oncology, Heidelberg University Hospital

Summary

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Keywords CRISPR

Loss Of Function Analysis

Cerebellar Granule Cells

In Utero Electroporation

Gene Transfer

Developmental Neuroscience

Neuro oncology

Cerebellar Development

Brain Tumor Formation

Chapters in this video

0:04

Title

1:30

In Utero Electroporation

5:16

Preparation of Cryosections from Electroporated Cerebella

6:22

Immunostaining the Cerebellar Cryosections

7:38

Results: Immunostained Cerebella from Rosa26-CAG-LSL-Cas9-P2A-EGFP Mouse

8:48

Conclusion

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