The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

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13.3K Views

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12:15 min

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October 3rd, 2017

DOI :

10.3791/56100-v

October 3rd, 2017

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Nan Zhang¹^,², Liakot A Khan¹, Edward Membreno¹, Gholamali Jafari¹, Siyang Yan¹, Hongjie Zhang¹^,³, Verena Gobel¹

¹Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital, Harvard Medical School, ²College of Life Sciences, Jilin University, ³Faculty of Health Sciences, University of Macau

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:24

Introduction to the Accompanying Publication

2:47

Nematode Fixation

4:19

Nematode Antibody Staining

6:33

Applying RNAi to Nematodes Using a Feeding Protocol

7:57

Analyzing Phenotypes Using Fluorescence Dissecting Microscopy

8:41

Analyzing Phenotypes Using Confocal Microscopy

10:14

Results: Immunohistochemistry, RNAi and Microscopic Analysis of Intestinal Apical membrane and Lumen Morphogenesis

11:45

Conclusion

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