JoVE Journal
Genetics
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Nanyang Technological University7.5K Views
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08:37 min
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April 30th, 2018
DOI :
April 30th, 2018
•0:04
Title
0:42
sgRNA Target Selection and Homology-directed Repair Template Design
2:21
Prepare Injection Mix and Injection Protocol
3:18
Screen P0 Plates and Single mCherry(+) F1s
4:03
Single Worm PCR and Genotyping
5:41
Indentification and Sequence Verification of Edited Animals
6:34
Results: Rapid CRISPR/Cas9 Generated Point Mutations in C. elegans sod-1
7:50
Conclusion
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ISSN 2578-6326
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