JoVE Journal
Biochemistry
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Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin
363 Views
•
10:19 min
•
January 24th, 2025
DOI :
January 24th, 2025
•Chapters in this video
0:00
Introduction
1:22
Preparation and Modification of Coverslips for Protein Immobilization in Chambers
4:12
Protein Biotinylation and Fluorophore Labeling for Single-Molecule Frster Resonance Energy Transfer (smFRET) Assays
6:41
Single-Molecule FRET Imaging and Data Analysis for GTP Hydrolysis Cycle of Atlastin
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ISSN 2578-2037
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