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Biochemistry
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Nanyang Technological UniversityUsing Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
2.5K Views
•
08:02 min
•
May 5th, 2022
DOI :
May 5th, 2022
•Chapters in this video
0:04
Introduction
0:40
Polydimethylsiloxane (PDMS) Chamber Assembly
1:45
Connect Microfluidic Device
3:11
Configuring the Setup with Standard Flow Rates and Changing Solution
4:21
Basic Single Filament Experiment: Adenosine Diphosphate (ADP)-Actin Barbed End Depolymerization
6:45
Results: Polymerization of Actin Filaments from Spectrin-Actin Seeds when Exposed to Different Actin Binding Proteins
7:24
Conclusion
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ISSN 2578-2037
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