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Nanyang Technological University

2D-HPLC-MS Technology Combined with Molecular Network for the Identification of Components in Tibetan Medicine Aconitum pendulum

427 Views

•

07:50 min

•

December 8th, 2023

DOI :

10.3791/66239-v

December 8th, 2023

•

Jing Ma*¹, Hanyu Lu*¹, Jia Liu¹, Ting Wang¹, Xing Fu², Xinmei Xu², Yi Zhang¹, Jing Zhang¹, Xiaolong Xie¹, Yingzhuang Chen³^,⁴, Jinsong Su¹

¹Ethnic Medicine Academic Heritage Innovation Research Center, Meishan Hospital, Chengdu University of Traditional Chinese Medicine, ²State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, ³Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, ⁴Key Laboratory of Phytochemical R&D of Hunan Province, Hunan Normal University

Summary

This study utilizes two-dimensional high-performance liquid chromatography-mass spectrometry (2D-HPLC-MS) technology in conjunction with molecular networking to unravel the intricate chemical composition of the Tibetan medicinal plant Aconitum pendulum Busch (APB). The article provides a detailed protocol for the systematic exploration and identification of complex chemical components of herbal medicines.

Explore More Videos

Molecular Networking

Tibetan Medicine

Aconitum Pendulum

Chemical Composition

Mass Spectrometry

Natural Medicines

Bioactive Molecules

Phytochemical Profile

Herbal Medicines

Chemical Constituents

Complex Mixtures

Therapeutic Properties

Natural Product Discovery

Chapters in this video

0:00

Introduction

1:40

Sample Preparation for 2D‐HPLC‐MS of Aconitum pendulum

3:21

Implementing 2D‐Liquid Chromatography on Aconitum pendulum Samples for Enhanced Compound Separation

4:26

Mass Spectrometry Following the 2D‐LC Operation on Aconitum pendulum Samples

5:47

Integration of Molecular Network Operation to 2D‐HPLC‐MS for the Analysis of Aconitum pendulum Samples

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