Generating Kidney Organoids in Suspension from Induced Pluripotent Stem Cells

2.2K Views

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07:22 min

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September 1st, 2023

DOI :

10.3791/65698-v

September 1st, 2023

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Langping Gao*¹^,², Yue Wang*³, Gang Wang⁴, Hangdi Wu², Qingtao Yan¹, Jingjing Wang¹, Fei Liu¹, Haidong Fu¹, Wei Li⁵, Lidan Hu¹, Jianhua Mao¹^,²

¹Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, ²Zhejiang University School of Medicine, ³Hubei Normal University, ⁴National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, ⁵Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health

Summary

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Kidney Organoids

Induced Pluripotent Stem Cells

Differentiation Ability

APEL 2 Medium

Fibroblast Growth Factor 9

Epigenetics

Hereditary Kidney Disease

Protocol Optimization

Chapters in this video

0:00

Introduction

2:31

iPSC Differentiation into Posterior Primitive Streak and Nephrogenic Intermediate Mesoderm

4:50

Generating Kidney Organoids from Induced Pluripotent Stem Cell Suspension Culture

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