Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles

1.8K Views

•

08:04 min

•

November 3rd, 2023

DOI :

10.3791/65259-v

November 3rd, 2023

•

Tiffany M. Richardson*¹, Yasminye D. Pettway*¹, John T. Walker¹, Heather A. Nelson², Matthew Ishahak³, Gregory Poffenberger², Radhika Aramandla², Conrad Reihsmann², Ashutosh Agarwal³, Alvin C. Powers¹^,²^,⁴, Marcela Brissova²

¹Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, ²Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, ³Department of Biomedical Engineering, University of Miami, ⁴VA Tennessee Valley Healthcare System

Summary

Explore More Videos

Pseudoislet System

Biosensors

Fluorescent Biosensor Dynamics

Beta Cell Dysfunction

Diabetes

Human Pancreas

Eyelid Biology

Gene Silencing Strategies

Intracellular Signaling Pathways

Live cell Imaging

Microperfusion System

Chapters in this video

0:00

Introduction

1:30

Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

Related Videos

19:16

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

15.9K Views

08:15

Tracking Dynamics of Muscle Engraftment in Small Animals by In Vivo Fluorescent Imaging

9.7K Views

09:58

A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid

20.7K Views

06:26

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA

11.9K Views

16:07

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

20.5K Views

10:19

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

11.3K Views

11:17

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

16.4K Views

09:35

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

17.7K Views

08:21

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

12.8K Views

09:27

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

11.1K Views