Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
2.6K Views
•
08:42 min
•
May 26th, 2023
DOI :
May 26th, 2023
•Chapters in this video
0:04
Introduction
0:47
Isolation of Bronchial Epithelial Cells from Human Lung Tissue
2:17
Cryopreservation of Human Primary Bronchial Epithelial Cells (PBECs)
3:28
Thawing Cryopreserved PBECs and Growing Them for Culture on Inserts
4:23
Establishing an Air‐Liquid Interface Culture with Primary Bronchial Epithelial Cells (ALI‐PBEC)
6:03
Results: Characterization of ALI Cultures Using Cell‐Specific Marker Genes
7:41
Conclusion
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