P. aeruginosa Infected 3D Co-Culture of Bronchial Epithelial Cells and Macrophages at Air-Liquid Interface for Preclinical Evaluation of Anti-Infectives
10.7K Views
•
10:26 min
•
June 15th, 2020
DOI :
June 15th, 2020
•Carlos Victor Montefusco-Pereira*1,2, Justus C. Horstmann*1,2, Thomas Ebensen3, Christoph Beisswenger4, Robert Bals4, Carlos A. Guzmán3, Nicole Schneider-Daum1, Cristiane de Souza Carvalho-Wodarz1, Claus-Michael Lehr1,2
1Department of Drug Delivery, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), 2Department of Pharmacy, Saarland University, 3Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, 4Department of Internal Medicine V - Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital
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Chapters in this video
0:04
Introduction
1:26
Growth and Differentiation of Cells in Permeable Support Inserts
5:29
Infection by P. aeruginosa
7:00
Measurement of Bacterial Proliferation via Colony-forming Units (CFU)
8:00
Results: Epithelial-macrophage Co-cultures Infected with P. aeruginosa
9:48
Conclusion
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