Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
1.1K Views
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09:02 min
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September 22nd, 2023
DOI :
September 22nd, 2023
•1Department of Microbiology, University of Pennsylvania, 2Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Perelman School of Medicine, University of Pennsylvania, 3Otorhinolaryngology-Head and Neck Surgery, University of Pennsylvania, 4Corporal Michael J. Crescenz VA Medical Center
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Chapters in this video
0:04
Introduction
0:57
Infection of Nasal Air‐Liquid Interface (ALI) Cultures
2:53
Collection and Quantification of the Shed Virus in Apical Surface Liquid (ASL)
3:49
Transepithelial Electrical Resistance (TEER) Measurement
5:25
Measuring Cytotoxicity by Lactate Dehydrogenase (LDH) Assay
6:18
Results: Characterizing Viral Replication, Host Cell Tropism, and Virus‐Induced Cytotoxicity in Nasal ALI Cultures
8:11
Conclusion
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