JoVE Journal

Biology

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Nanyang Technological University

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

We present a protocol that combines recombinase polymerase amplification with a CRISPR/Cas12a system for trace detection of DNA viruses and builds portable smartphone microscopy with an artificial intelligence-assisted classification for point-of-care DNA virus detection.

Chapters in this video

0:00

Introduction

0:40

Combining Recombinase Polymerase Amplification (RPA) and CRISPR/Cas12a Systems for the Detection of DNA Viruses

1:20

CRISPR/Cas12a Detection With/Without Smartphone Microscopy (SPM)

2:23

Data Augmentation and Transfer Learning

2:58

Results: AI-Detection of DNA Virus Using RPA-CRISPR/Cas12a-SPM

3:26

Conclusion

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