Reusable Single Cell for Iterative Epigenomic Analyses
1.2K Views
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10:28 min
•
February 11th, 2022
DOI :
February 11th, 2022
•1Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 2Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 3CCR Collaborative Bioinformatics Resource (CCBR), Center for Cancer Research, National Cancer Institute, National Institutes of Health, 4Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute
Chapters in this video
0:05
Introduction
1:32
Preparation of Reusable Single Cells: Manual Version
3:09
Random Primer Annealing and Extension
4:09
Antibody Binding and Proximity Ligation of Antibody Probe and Proximally Extended Random Primer
5:25
Full Extension of the 1st Random Primer and Multiple Displacement Amplification
7:32
Results: Single Cell Multiepigenomic Analysis
9:26
Conclusion
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