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Developmental Biology

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Nanyang Technological University

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

4.0K Views

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09:48 min

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February 25th, 2022

DOI :

10.3791/63431-v

February 25th, 2022

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Emma Laporte*¹, Charlotte Nys*¹, Hugo Vankelecom¹

¹Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute, KU Leuven (University of Leuven)

Summary

The pituitary gland is the key regulator of the body's endocrine system. This article describes the development of organoids from the mouse pituitary as a novel 3D in vitro model to study the gland's stem cell population of which the biology and function remain poorly understood.

Explore More Videos

Mouse Pituitary

Stem Cell Biology

Pituitary Remodeling

Neonatal Maturation

Functional Decline

Organoid Technique

Trypsin Solution

Cell Suspension

Chapters in this video

0:04

Introduction

0:53

Isolation of Mouse Anterior Lobe

1:57

Dissociation of Pituitary into Single Cells

4:28

Establishment and Culturing of Anterior Lobe Derived Organoids

7:31

Results: Establishment and Validation of Anterior Lobe Derived Organoids

9:21

Conclusion

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