Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
5.4K Views
•
10:16 min
•
December 11th, 2021
DOI :
December 11th, 2021
•Artem Eremeev1,3, Lilia Belikova1,2, Evgeny Ruchko1, Egor Volovikov1,5, Olga Zubkova1, Alexy Emelin4, Roman Deev4, Olga Lebedeva1,3, Alexandra Bogomazova1,3, Maria Lagarkova1,3
1Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 4Department of Pathological Anatomy, North-Western State Medical University, 5Department of Biology, Lomonosov State University
Chapters in this video
0:02
Introduction
1:41
Transforming Petri Dishes Into Mini Bioreactors
2:51
Induction of Neuronal Differentiation of iPSCs
3:51
The Formation of Spheroids from Neuroepithelial Precursor Cells
5:38
Obtaining and Cultivation of Organoids
7:59
Results
9:19
Conclusion
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