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Bioengineering

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Nanyang Technological University

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

3.7K Views

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13:04 min

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January 18th, 2022

DOI :

10.3791/63401-v

January 18th, 2022

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Giuseppe Ciccone¹, Mariana Azevedo Gonzalez Oliva¹, Nelda Antonovaite², Ines Lüchtefeld³, Manuel Salmeron-Sanchez¹, Massimo Vassalli¹

¹Centre for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, ²Optics 11 life, ³Laboratory of Biosensors and Bioelectronics, ETH Zürich

Summary

The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. First, the experimental steps to acquire force spectroscopy data are detailed; then, the analysis of such data is detailed through a newly developed open-source Python software, which is free to download from GitHub.

Explore More Videos

Nanoindentation

Mechanical Properties

Atomic Force Microscopy

Data Analysis Workflow

Calibration Protocol

Commercially Available Nanoindenter

Open source Software

Probe Calibration

Microscope Software

Equilibrium Conditions

Calibration Factors

Chapters in this video

0:05

Introduction

0:43

Probe Calibration

3:38

Measuring the Young s Modulus of Soft Materials

5:30

Data Analysis

6:33

Formal Data Analysis

10:08

Results: Acquiring Nanoindentation Data on Hydrogels and Cells

12:26

Conclusion

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