Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

3.6K Views

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05:29 min

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June 9th, 2021

DOI :

10.3791/62421-v

June 9th, 2021

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Delphine M. Pott¹, José G. Vallarino¹, Sonia Osorio¹

¹Departamento de Biología Molecular y Bioquímica, Campus de Teatinos, Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga-Consejo Superior de Investigaciones Científicas

Summary

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Volatile Compounds

Blackcurrant

Headspace Solid phase Microextraction

Gas Chromatography mass Spectrometry

Fruit Aroma

Breeding Programs

VOC Extraction

Sodium Chloride Solution

Internal Standard

Automated HS SPME GC MS

Retention Times

Mass Spectra

Kovats Linear Retention Indexes

Peak Area Calculation

Batch Effect Correction

Chapters in this video

0:05

Introduction

0:42

Sample Preparation

1:17

Headspace Solid-phase Microextraction (HS-SPME) Coupled with Gas Chromatography-mass Spectrometry (GC-MS) Data Acquisition

2:23

Analysis of GC-MS Profile Chromatograms: VOC Identification and Semi-quantification

3:20

Results: Analysis of HS-SPME/GC-MS Chromatograms, PCA, and Relative VOC Content in Blackcurrant Fruits

4:48

Conclusion

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