Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
11.8K Views
•
10:37 min
•
November 15th, 2017
DOI :
November 15th, 2017
•Chapters in this video
0:05
Title
1:00
Preparation of Cells/Tissues
1:45
Protein Extraction, Quality Control Western Blotting, In-solution Digestion, and Peptide Desalting
4:45
TMT Labeling of Peptides
5:50
Extensive High-resolution, Basic pH LC Prefactionation
6:43
LC-MS/MS Preparation and Parameters
7:41
Results: Deep Proteome Profiling Analysis
8:45
Conclusion
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