Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

9.8K Views

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09:18 min

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April 17th, 2017

DOI :

10.3791/55464-v

April 17th, 2017

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Cristina Lento¹, Shaolong Zhu¹, Kerene A. Brown¹, Ruth Knox¹, Peter Liuni¹, Derek J. Wilson¹^,²^,³

¹Department of Chemistry, York University, ²The Centre for Research in Mass Spectrometry, York University, ³The Centre for Research on Biomolecular Interactions, York University

Summary

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Keywords Time resolved Electrospray Ionization

Hydrogen deuterium Exchange

Mass Spectrometry

Protein Structure

Protein Dynamics

Conformational Flexibility

Transient Protein Conformations

Loop Regions

Molten Globules

Intrinsically Disordered Proteins

Neurodegenerative Disorders

Protein Misfolding

Protein Aggregation

Enzyme Catalytic Turnover

Protein protein Interactions

Protein ligand Interactions

Continuous Flow

Time resolved Kinetic Mixer

Orthogonal Mixing

Chapters in this video

0:05

Title

1:12

Preparation of the Microfluidic Device

5:17

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange

6:51

Results: Detection of Protein Structural Changes by Time-resolved ElectroSpray Ionization Hydrogen-deuterium Mass Spectrometry

8:10

Conclusion

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