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Nanyang Technological UniversityIsolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
4.7K Views
•
07:35 min
•
April 5th, 2021
DOI :
April 5th, 2021
•Inn Chuan Ng*1, Li Zhang*2, Narelle Nichola Yi Ying Shen3, Yun Ting Soong4, Chan Way Ng5, Phoebe Kang Sheing Koh1, Yan Zhou3, Hanry Yu1,3,4,5,6
1Department of Physiology & The Institute for Digital Medicine (WisDM), National University of Singapore, 2College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute, National University of Singapore, 4Institute of Bioengineering and Nanotechnology, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP, Singapore-MIT Alliance for Research and Technology
Chapters in this video
0:04
Introduction
0:45
Portal Vein Cannulation
3:54
Liver Perfusion, Digestion, Hepatocyte Isolation
5:30
Results: Estimation of Purity and Function of Isolated Hepatocytes
7:07
Conclusion
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