JoVE Journal
Biology
This content is Open Access.
Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)
10.5K Views
•
09:25 min
•
May 8th, 2020
DOI :
May 8th, 2020
•Chapters in this video
0:04
Introduction
0:49
Tamoxifen (TM) Induction in Mosaic Analysis with Double Markers (MADM) Mice
2:00
MADM Clone Brain Tissue Harvest
3:12
MADM Brain Tissue Sectioning
4:30
Adult Tissue Mounting
5:29
Confocal Image Acquisition and MADM Clone Quantification
6:29
Serial 3D Clone Reconstruction
7:30
Results: Representative Lineage Tracing and Clonal Analysis
8:41
Conclusion
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