JoVE Logo

Sign In

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

11.2K Views

09:27 min

May 6th, 2020

DOI :

10.3791/61090-v

May 6th, 2020


Explore More Videos

Multicellular Alveolar Model

Chapters in this video

0:04

Introduction

1:11

Peripheral Blood Monocytes (PBM) Isolation

3:07

CD14+ Cell Selection

4:00

PBM Seeding and Differentiation

4:46

Human Alveolar Epithelial Tissue Triple Cell Coculture Model

5:22

MDDC and MDM Seeding

7:03

Coculture Model Transfer to Air-Liquid Interface

7:30

Results: Representative Multicellular Human Alveolar Model Hazard Assessment

8:52

Conclusion

Related Videos

article

05:44

In vitro Cell Culture Model for Toxic Inhaled Chemical Testing

10.7K Views

article

10:10

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

18.9K Views

article

07:02

Generating 3D Spheres and 2D Air-Liquid Interface Cultures of Human Induced Pluripotent Stem Cell-Derived Type 2 Alveolar Epithelial Cells

4.6K Views

article

12:04

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

13.6K Views

article

06:27

iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease

771 Views

article

08:42

Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures

2.6K Views

article

10:10

Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface

6.9K Views

article

10:30

Immunocompetent Alveolus-on-Chip Model for Studying Alveolar Mucosal Immune Responses

1.1K Views

article

06:02

Isolation of Live Myeloid and Epithelial Cell Populations from the Mouse Lung

428 Views

article

09:29

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

11.3K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved