JoVE Journal

Developmental Biology

You have full access to this content through

Nanyang Technological University

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

6.6K Views

•

06:49 min

•

October 29th, 2019

DOI :

10.3791/60362-v

October 29th, 2019

•

Renat N. Khaliullin¹^,²^,³, Jeffrey M. Hendel¹^,², Adina Gerson-Gurwitz¹^,², Shaohe Wang¹^,⁴^,⁵, Stacy D. Ochoa¹^,⁶, Zhiling Zhao¹^,⁷, Arshad Desai¹^,², Karen Oegema¹^,², Rebecca A. Green¹^,²

¹Ludwig Institute for Cancer Research, San Diego, ²Department of Cellular and Molecular Medicine, University of California, San Diego, ³Recursion Pharmaceuticals, ⁴Biomedical Sciences Graduate Program, University of California, San Diego, ⁵Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, ⁶Department of Biology, San Diego State University, ⁷Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco

Summary

This work describes a semi-high-throughput protocol that allows simultaneous 3D time-lapse imaging of embryogenesis in 80–100 C. elegans embryos in a single overnight run. Additionally, image processing and visualization tools are included to streamline data analysis. The combination of these methods with custom reporter strains enables detailed monitoring of embryogenesis.

Explore More Videos

Embryonic Development

Semi high throughput Imaging

3D Time Lapse Imaging

Quantitative Analysis

Developmental Processes

Imaging Protocol

Sample Preparation

Confocal Microscope

Embryo Transfer

Dissection Technique

Temperature controlled Environment

Chapters in this video

0:04

Title

1:01

C. elegans Embryo Semi-High-Throughput Imaging Preparation

3:15

Automated Cropping

4:12

Visualization

5:11

Results: Representative High-Content Imaging of C. elegans Embryogenesis

5:56

Conclusion

Related Videos

article

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

9.0K Views

article

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

7.7K Views

article

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

10.1K Views

article

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

11.3K Views

article

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

6.4K Views

article

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

8.4K Views

article

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

7.5K Views

article

Immobilization of Caenorhabditis elegans to Analyze Intracellular Transport in Neurons

8.0K Views

article

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

5.9K Views

article

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

6.2K Views

Copyright © 2025 MyJoVE Corporation. All rights reserved

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More