JoVE Journal

Developmental Biology

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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.

Chapters in this video

0:05

Title

0:48

Embryo Collection for Time-lapse Imaging

1:51

Embryo Preparation

2:52

Preparation of 2% Low-melt Agarose and Mounting the Embryo

4:34

Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition

7:26

Post-acquisition Processing

8:10

Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos

9:29

Conclusion

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