Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
11.9K Views
•
09:49 min
•
October 11th, 2019
DOI :
October 11th, 2019
•Cristina Melero*1, Aljona Kolmogorova*1, Paul Atherton1, Brian Derby2, Adam Reid3,4, Karin Jansen5, Christoph Ballestrem1
1Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research, The University of Manchester, 2School of Materials, The University of Manchester, 3Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester, Manchester Academic Health Science Centre, 4Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, 5Department of Pathology, UMC Utrecht
Chapters in this video
0:04
Title
0:57
Design of Pattern Templates and Plasma Cleaning
1:52
Passivation with PLL-PEG
2:54
System Calibration
4:36
Software Set-up and Photopatterning
5:29
Protein Incubation and Blocking Unspecific Binding Sites
6:43
Second Round of Photopatterning and Protein Incubation
7:39
Results: Micropatterning for the Study of Cell Responses to Extracellular Matrix Proteins
9:03
Conclusion
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