Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
30.3K Views
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10:47 min
•
November 6th, 2019
DOI :
November 6th, 2019
•Vincent van Duinen1,2, Wendy Stam1, Viola Borgdorff3, Arie Reijerkerk3, Valeria Orlova4, Paul Vulto5, Thomas Hankemeier2, Anton Jan van Zonneveld1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas
Chapters in this video
0:04
Title
1:12
Preparation of Device, Gel, and Coating
2:42
Cell Seeding/Microvessel Culture
4:37
Study Angiogenic Sprouting
5:49
Study Anastomosis and Sprout Stabilization
6:50
Fixation, Staining, and Imaging
8:55
Results: Cell Seeding and 3D Sprouting of iPSC-EC
10:13
Conclusion
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