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Nanyang Technological University

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

2.8K Views

•

08:04 min

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May 11th, 2021

DOI :

10.3791/62554-v

May 11th, 2021

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Georgios Galaris¹, Jérémy H. Thalgott¹, Eliott Teston¹, Franck P.G. Lebrin¹^,²^,³

¹Einthoven Laboratory for Experimental Vascular Medicine, Department of Internal Medicine (Nephrology), Leiden University Medical Center, ²Institute Physics for Medicine Paris, INSERM U1273, ESPCI Paris, CNRS FRE 2031, ³MEMOLIFE Laboratory of Excellence and PSL Research University

Summary

This assay utilizes mouse embryonic stem cells differentiated into embryoid bodies cultured in 3D-collagen gel to analyze the biological processes that control sprouting angiogenesis in vitro. The technique can be applied for testing drugs, modeling diseases, and for studying specific genes in the context of deletions that are embryonically lethal.

Explore More Videos

Three dimensional Sprouting Assay

Mouse Embryonic Stem Cells

Vascular Disease Modeling

Endothelial Cells

Genetic Screening

Mesoderm Differentiation

Pathological Angiogenesis

Cell Suspension

Chapters in this video

0:04

Introduction

1:14

Embryoid Bodies (EB) Formation in Hanging Drop

3:02

Floating EBs Culture for Vascular Differentiation

3:41

3D Sprouting Angiogenesis Assay and Immunofluorescence Staining

5:44

Results: Microscopic Analysis and Quantification of Mouse EBs Undergoing Angiogenesis Assay

7:29

Conclusion

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