Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

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30.3K Views

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10:47 min

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November 6th, 2019

DOI :

10.3791/59678-v

November 6th, 2019

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Vincent van Duinen¹^,², Wendy Stam¹, Viola Borgdorff³, Arie Reijerkerk³, Valeria Orlova⁴, Paul Vulto⁵, Thomas Hankemeier², Anton Jan van Zonneveld¹

¹Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, ²Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, ³Ncardia, ⁴Department of Anatomy and Embryology, Leiden University Medical Center, ⁵Mimetas

Podsumowanie

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Angiogenic Sprouting

IPSC derived Endothelial Cells

In Vitro Assay

High Throughput Screening

Microfluidic Platform

Angiogenesis Mechanisms

Cardiovascular Diseases

Tissue Regeneration

Collagen Solution

Fibronectin Coating

Microfluidic Channels

Pipetting Techniques

Sterile Laminar Flow Hood

Rozdziały w tym wideo

0:04

Title

1:12

Preparation of Device, Gel, and Coating

2:42

Cell Seeding/Microvessel Culture

4:37

Study Angiogenic Sprouting

5:49

Study Anastomosis and Sprout Stabilization

6:50

Fixation, Staining, and Imaging

8:55

Results: Cell Seeding and 3D Sprouting of iPSC-EC

10:13

Conclusion

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