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High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

8.8K Views

13:27 min

August 8th, 2019

DOI :

10.3791/59570-v

August 8th, 2019


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High throughput

Chapters in this video

0:04

Title

0:47

Preparation of the Peristaltic Liquid Handler Programs

1:53

Experimental Design and Generation of the Picklists to Drive the Acoustic Droplet Ejection (ADE)-Based Dispenses

3:34

DNA Source Plate Preparation Using the 384-well Pipetting Guide Application and Survey Performance to Control the Manually Dispensed Volumes

6:18

Peristaltic Liquid Handler-Based 1 μL Diluent Dispensation in the Destination Plate

7:03

ADE-driven DNA Dispensing into the Destination Plate and ADE-driven Transfection Reagent Dispensing

9:54

Peristaltic Liquid Handler-Based Cell Dispensation

10:51

Results: Defining the Key Parameters to Reach Highly Efficient and High Thoughput Cells (Co-) Transfections

12:26

Conclusion

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