Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
8.9K Views
•
10:13 min
•
May 6th, 2019
DOI :
May 6th, 2019
•Chapters in this video
0:04
Title
1:14
T Antigen (Ag)-Expressing Tumor Cell Inoculation
2:29
Target Splenocyte Preparation
4:10
Target Splenocyte Coating with Irrelevant and Cognate Peptides and Carboxyfluorescein Succinimidyl Ester (CFSE) Labeling
5:44
CFSE-Labeling Evaluation
6:50
CFSE-Labelled Target Cell Injection into Nave and T-Ag-Primed Recipients
7:31
Data Acquisition
8:21
Results: Representative Analyses of CD8+ T Cell-Mediated Cytotoxicity
9:36
Conclusion
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