JoVE Journal

Immunology and Infection

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Nanyang Technological University

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

Chapters in this video

0:05

Title

1:00

Administration of the Immunostimulatory Cocktail Covax

1:57

Isolation of Target Splenocytes for Labeling with CFSE and Peptide Pulsing of Target Splenocytes

2:55

Preparation of CFSE and Labeling of Target Splenocytes

4:52

Results: Detection of Antigen-specific T-cell Cytolytic Function

6:20

Conclusion

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