Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
9.3K Views
•
09:50 min
•
August 14th, 2019
DOI :
August 14th, 2019
•Zhiping Zhang1, Shuhang Fan1, Zhiqiang Chen1, Yanzhi He1, Mengfei Huang1, Li Ding1, Yanyan Zhang5, Lin Chen1, Xinyue Zhang1,2,3,4
1College of Bioscience and Biotechnology, Yangzhou University, 2Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, 3Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, 4Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 5The Testing Center, Yangzhou University
Chapters in this video
0:04
Title
1:13
Produce a Flavonol from a Flavanone in an in vitro Bienzyme Synthetic System
2:48
Thin Layer Chromatography (TLC) Analysis
5:21
High Performance Liquid Chromatography (HPLC) and Liquid Chromatography/ Mass Spectrometry (LC/MS) Analyses
7:50
Results: Conversion of Flavanone into Flavono
9:16
Conclusion
Related Videos
10:49
Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
12.5K Views
06:34
A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
11.8K Views
06:17
Measuring Protein Binding to F-actin by Co-sedimentation
16.4K Views
06:44
Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups
9.6K Views
10:01
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
12.1K Views
14:23
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
13.3K Views
11:54
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
9.3K Views
08:43
Detergent-assisted Reconstitution of Recombinant Drosophila Atlastin into Liposomes for Lipid-mixing Assays
7.6K Views
06:26
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
3.1K Views
09:09
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
2.9K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved