Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
8.8K Views
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11:47 min
•
April 3rd, 2019
DOI :
April 3rd, 2019
•Hannah C. Leeson1,2, Tailoi Chan-Ling3,4, Michael D. Lovelace3,5,6, Jeremy D. Brownlie7, Michael W. Weible II*1,4,7, Ben J. Gu*8
1Griffith Institute for Drug Discovery, Griffith University, 2Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, 4Bosch Institute, University of Sydney, 5Applied Neurosciences Program, Peter Duncan Neurosciences Research Unit, St. Vincent's Centre for Applied Medical Research, 6School of Medical Sciences, The University of New South Wales (UNSW) Medicine, Sydney, New South Wales, 7School of Environment and Science, Griffith University, Brisbane, Queensland, 8Florey Institute of Neuroscience and Mental Health, University of Melbourne
Chapters in this video
0:04
Title
0:58
Preparation of a Single-cell Suspension of Neural Progenitor Cells for Analysis by Flow Cytometry
2:11
Measuring Calcium Influx by Live-cell Flow Cytometry
5:21
Measuring Pore Formation by Live-cell Flow Cytometry
7:02
Measuring Phagocytosis by Live-cell Flow Cytometry
9:02
Results: Analyses of Calcium Influx, Pore Formation, and Phagocytosis by Live-cell Flow Cytometry
10:46
Conclusion
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