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Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

7.3K Views

11:50 min

March 18th, 2019

DOI :

10.3791/59282-v

March 18th, 2019


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Stem Cell Isolation

Chapters in this video

0:04

Title

0:47

Tooth and Dental Pulp Extraction

1:29

Processing of the Dental Pulp and Stem Cell Release

2:29

Stem Cell Culture and Propagation

3:44

Transient PrPC Silencing by siRNA

5:04

Neuronal Induction Process of hDPSCs

5:50

Characterization of hDPSCs by Flow Cytometry

8:03

Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis

9:14

Results: hDPSCs Isolation and Propagation and the Role of PrP During Neuronal Differentiation

10:54

Conclusion

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