JoVE Journal
Immunology and Infection
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Nanyang Technological UniversityHigh Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
5.7K Views
•
07:18 min
•
January 22nd, 2019
DOI :
January 22nd, 2019
•Chapters in this video
0:04
Title
0:32
Transfection of HEK293T Cells with Dual Color Reporter Construct
2:01
Treatment of Transfected HEK293T Cells with Latency Reversing Agents
2:33
Staining of Transfected Cells with Fixable Viability Dye for Flow Cytometry Analysis
4:40
EGFP and DsRed Measurements by Flow Cytometry and Data Analysis
5:49
Results: Assessment of Latency Reversing Agents on HIV Transcription and Splicing
6:43
Conclusion
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ISSN 2689-3649
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