Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

7.4K Views

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10:34 min

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February 22nd, 2017

DOI :

10.3791/54581-v

February 22nd, 2017

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Muthukumar Balasubramaniam¹^,², Benem Davids¹^,², Amma B. Addai¹^,², Jui Pandhare¹^,³^,⁴, Chandravanu Dash¹^,²^,⁵

¹Center for AIDS Health Disparities Research, Meharry Medical College, ²Department of Biochemistry and Cancer Biology, Meharry Medical College, ³School of Graduate Studies and Research, Meharry Medical College, ⁴Department of Microbiology and Immunology, Meharry Medical College, ⁵Tennessee Center for AIDS Research (TN-CFAR), Meharry Medical College

Summary

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Keywords HIV 1

Preintegration Complexes

Antiretroviral Therapy

Chapters in this video

0:05

Title

1:36

Virus Infection of Sup-T1 Cells

3:22

Harvesting and Lysis of Infected Cells

4:22

Preparation of Cytoplasmic Preintegration Complexes

5:32

In Vitro Integration Assay Using Preintegration Complexes

7:40

PCR Assays for Quantifying Preintegration Complex Activity

8:29

Results: HIV-1 Preintegration Complex-associated vDNA Integration Activity

9:47

Conclusion

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